利用放射免疫技术快速检测转基因植物

利用PCR分别合成掺入地高辛标记的35S启动子和NOS终止子核酸探针，再利用放射免疫技术将I^125标记的地高辛抗体与35S启动子和NOS终止子探针分别进行杂交检测。结果显示转基因样本的杂交信号均为阳性，且灵敏度较高。特异性强，为转基因植物的分析检测又提供了一种较实用的方法。     

改性坡缕石对肉鸭日粮中黄曲霉毒B1的去毒作用及机理

通过体外和体内试验研究了改性坡缕石对肉鸭日粮中黄曲霉毒素B1的去毒作用.体外试验表明与坡缕石相比,改性坡缕石对黄曲霉毒素B1的吸附率更强.480羽1日龄樱桃谷商品代肉鸭,按饲养试验要求随机分为4组1)对照组为基础日粮;2)基础日粮+0.25%改性坡缕石;3)霉变玉米50%代替基础日粮中的玉米,使黄曲霉毒素B1前期为50μg/kg,后期为55μg/kg;4)第3组日粮+0.25%改性坡缕石.结果表明与对照组相比,添加0.25%改性坡缕石对肉鸭生长性能、组织和血清黄曲霉毒素B1含量、内脏器官相对质量、血清生化指标均无显著影响.然而,霉变玉米使肉鸭生长性能显著降低,使胸肌、肝脏、肾脏、血清黄曲霉毒素B1含量以及肝脏率、肾脏率、胰脏率、血清谷草转氨酶、谷丙转氨酶、淀粉酶水平显著提高;使血清总蛋白、白蛋白、尿酸水平显著降低.联合饲喂霉变玉米/改性坡缕石可使肉鸭生长性能、组织器官和血清黄曲霉毒素B1含量、内脏器官相对质量、血清生化指标均恢复至对照组水平.上述结果提示,改性坡缕石对肉鸭日粮中黄曲霉毒素B1具有较好的去毒作用.     

Biocontrol strain Pseudomonas fluorescens CHA0 and its genetically modified derivative with enhanced biocontrol capability exert comparable effects on the structure of a Sinorhizobium meliloti population in gnotobiotic systems

The impact of biocontrol strain Pseudomonas fluorescens CHA0 and of its genetically modified, antibiotic-overproducing derivative CHA0/pME3424 on a reconstructed population of the plant-beneficial Sinorhizobium meliloti bacteria was assessed in gnotobiotic systems. In sterile soil, the final density of the reconstructed S. meliloti population decreased by more than one order of magnitude in the presence of either of the Pseudomonas strains when compared to a control without addition of P. fluorescens. Moreover, there was a change in the proportion of each individual S. meliloti strain within the population. Plant tests also revealed changes in the nodulating S. meliloti population in the presence of strains CHA0 or CHA0/pME3424. In both treatments one S. meliloti strain, f43, was significantly reduced in its root nodule occupancy. Analysis of alfalfa yields showed a slight but statistically significant increase in shoot dry weight when strain CHA0 was added to the reconstructed S. meliloti population whereas no such effect was observed with CHA0/pME3424.     

转基因大豆种子、豆粕定性PCR检测方法的研究与应用

利用定性PCR检测方法,以大豆凝集素基因(Lectin)为内标基因,检测了转基因大豆种子、豆粕中的四个外源基因:35S-CTP基因、Cp4-epsps基因、nos终止子基因、CaMV35S启动子基因,并通过酶切(XmnI)验证试验验证了定性PCR检测结果的准确性,建立了定性PCR检测转基因大豆种子、豆粕的方法,利用市场上抽检到的大豆种子、豆粕样品,进行了实际检测工作.     

改性碱木质素产品作为牺牲剂在三次采油中的应用研究

研究了改性碱木质素HML和各种石油磺酸钠的吸附特性，以及作为三次采油牺牲剂减少石油磺酸钠吸附损失的作用规律，获得了改性碱木质素与大庆四厂原油产生超低界面张力的条件。结果表明HML作为牺牲剂可以显著地减少主表面活性剂石油磺酸钠ORS-41的吸附损失(减少量＞50％），HML能和石油磺酸钠、碱、聚合物配合将油水界面张力降至超低范围。改性碱木质素作为三次采油牺牲剂具有广泛的应用前景。     

兽用基因工程生物制品研究与开发的生物安全探讨措施

本文参照我国现行的一些法规，结合发达国家的一些经验，对动物用基因工程生物制品在研制与开发过程中应采取的完全控制措施进行了探讨。     

Combination Forecasting Models Using Regression Analysis Method

Using regression analysis method,the methods for solving the weights of combination forecasting model(CFM) are proposed. At first, the linear regress CFM are presented based on the least absolute criteria and least square criteria. Then the weights can be evaluated using the least square princinple. Because the objective function of CFM based on least absolute criteria is non differential, the traditional programming methods can not solve it. So the least square method with the modified weights is proposed to solve this problem. At the same time, methods for solving CFM is given with the aim of minimizing sum of percentage error absolutes. From many cases, the results show that the forecasting precision of CFM is very high and the effect of regression is remarkable.     

Differences of the Intestinal Microbial Flora Diversity and Composition in IGF-1 Transgenic Superfine Wool Sheep and Non-transgenic Sheep

The study aimed to explore the intestinal microbial community changes after transfering the IGF-1 gene into the superfine wool sheep, which would lead to potential problems in the biosafety of transgenic sheep. Forty one individuals in IGF-1 transgene positive group (GP group, female(GPF) 24 and male(GPM) 17), 43 individuals in genetically modified negative group (GN group, female(GNF) 25 and male(GNM) 18) and 29 individuals in non-genetically modified group(NG group, female(NGF) 18 and male(NGM) 11) from clinical healthy sheep in the same field were randomly selected. The rectal fecal samples were collected. The Ⅱlumina HiSeq high-throughput sequencing technology was used to determine bacterial 16S rRNA V3-V4 area sequence, and the bacteria community composition in fecal samples of transgenic superfine wool sheep and non-transgenic sheep was analyzed. There were 17 phyla, 32 classes, 56 orders, 94 families, 228 genera and 185 species. LEfSe analysis showed that there were 10 biomarkers in the GPF group, there were 5 biomarkers in GPM group, all of which were common colonization bacteria of ruminant intestinal flora. The biomarkers in NGF group was Bacteroides BS11 family, the biomarkers in NGM group was Firmicutes. They were the main dominant bacteria of ruminant intestinal flora. Analysis of shared bacteria of intestinal flora in 3 groups showed that the proportion of shared bacteria was as high as 91%-94% in 6 taxa of genus and family. It was confirmed that the introduction of IGF-1 gene did not change the overall distribution of intestinal flora in superfine wool sheep, and the transgenic sheep were biosafety and environmental safety.     

改良千里光注射液的体内外抗菌作用及安全性

分别用3种不同浓度乙醇进行醇提水沉法渗漉，制备了千里光原液。然后经高浓度乙醇法除去蛋白质、醇溶液调pH值法除去鞣质以及乙醚脱色等步骤进行除杂脱色，制备改良千里光注射液。对改良千里光注射液进行安全性检验、体外抑菌和体内抗感染试验，结果表明，用78％乙醇提取、制备的改良千里光注射液对肠炎沙门氏菌、大肠埃希氏菌、金黄色葡萄球菌、溶血性链球菌均有明显的抑制作用，体内抗感染作用显著，而且具有很好的澄明度和安全性。